Gorilla Grodd's time displacement machine transports many of Batman's worst enemies to feudal Japan, along with the Dark Knight and a few of his allies.

Set after the events of Continental Drift, Scrat's epic pursuit of his elusive acorn catapults him outside of Earth, where he accidentally sets off a series of cosmic events that transform and threaten the planet. To save themselves from peril, Manny, Sid, Diego, and the rest of the herd leave their home and embark on a quest full of thrills and spills, highs and lows, laughter and adventure while traveling to exotic new lands and encountering a host of colorful new characters.

Bugs Bunny and Daffy Duck are up to their feuding ways again. Tired of playing second fiddle to Bugs, Daffy has decided to leave the Studio for good. He is aided by Warner Bros.' humor impaired Vice President of Comedy, Kate Houghton, who releases him from his contract and instructs WB security guard/aspiring stunt man DJ Drake to capture and "escort" Daffy off the studio lot.

The world is under attack by an alien armada led by the powerful Apokoliptian, Darkseid. A group of superheroes consisting of Superman, Batman, Wonder Woman, The Flash, Green Lantern, Cyborg, and Shazam must set aside their differences and gather together to defend Earth.